Composite

Part:BBa_K2581019

Designed by: Laura Sans Comerma   Group: iGEM18_UPF_CRG_Barcelona   (2018-10-09)


FadE gene with ATC inducible promoter with weak RBS.

The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA dehydrogenase (fadE) gene which converts acyl-coA into enoyl-coA. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the fadE gene obtained of the own E.Coli DH5-alpha strain genome (BBa_K2581014, being a twin of BBa_k861020, a part not available).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 551
    Illegal SapI.rc site found at 1424
    Illegal SapI.rc site found at 2567


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